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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Solos. |
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Data corrente: |
06/02/2015 |
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Data da última atualização: |
06/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEITE, D. C. A.; BALIEIRO, F. C.; C. A. PIRES; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. C.; PEIXOTO, R. S. |
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Afiliação: |
D. C. A. LEITE, UFRJ; FABIANO DE CARVALHO BALIEIRO, CNPS; C. A. PIRES; BEATA EMOKE MADARI, CNPAF; A. S. ROSADO, UFRJ; HEITOR LUIZ DA COSTA COUTINHO, CNPS; R. S. PEIXOTO, UFRJ. |
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Título: |
Comparison of DNA extraction protocols for microbial communities from soil treated with biochar. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 175-183, 2014. |
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DOI: |
10.1590/S1517-83822014000100023 |
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Idioma: |
Inglês |
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Conteúdo: |
Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples. MenosMany studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-... Mostrar Tudo |
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Palavras-Chave: |
Comunidades microbianas; Extração de DNA; Índice de pureza do DNA; PCR-DGGE. |
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Thesaurus Nal: |
biochar. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117433/1/BJMv45n1a23.pdf
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Marc: |
LEADER 02342naa a2200265 a 4500
001 2007986
005 2015-02-06
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1590/S1517-83822014000100023$2DOI
100 1 $aLEITE, D. C. A.
245 $aComparison of DNA extraction protocols for microbial communities from soil treated with biochar.$h[electronic resource]
260 $c2014
520 $aMany studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples.
650 $abiochar
653 $aComunidades microbianas
653 $aExtração de DNA
653 $aÍndice de pureza do DNA
653 $aPCR-DGGE
700 1 $aBALIEIRO, F. C.
700 1 $aC. A. PIRES
700 1 $aMADARI, B. E.
700 1 $aROSADO, A. S.
700 1 $aCOUTINHO, H. L. C.
700 1 $aPEIXOTO, R. S.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 45, n. 1, p. 175-183, 2014.
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Embrapa Solos (CNPS) |
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